Compound_ID |
Target |
Gene_Name |
Gene_ID |
Cancer |
Cell_lines |
IC50 Value |
EC50 Value |
ED50 Value |
GI50 Value |
Remarks |
References |
MMP-AC0019 |
BAX |
BCL2-associated X protein |
581 |
Gastric adenocarcinoma |
SG7901 |
- |
- |
- |
- |
Genistein 0.5, 1 and 1.5 mg/kg significantly inhibited carcinoma growth when it was injected near the tumor by 10.8%, 29.9% and 39.6%, respectively. The apoptosis index was increased progressively with increasing genistein dose (28.9% ± 1.2%, 33.8% ± 1.6% and 37.7% ± 1.2%) . The positive rate of Bcl-2 protein was decreased progressively (11.9% ± 0.9%, 5.9% ± 0.7% and 4.2% ± 0.6%) by immunohistochemical staining, with increasing dose of genistein. The density of Bcl-2 mRNA decreased progressively with elongation of time by RT-PCR. |
18203299 |
MMP-AC0019 |
BCL2 |
B-cell CLL/lymphoma 2 |
596 |
Gastric adenocarcinoma |
SG7901 |
- |
- |
- |
- |
Genistein 0.5, 1 and 1.5 mg/kg significantly inhibited carcinoma growth when it was injected near the tumor by 10.8%, 29.9% and 39.6%, respectively. The apoptosis index was increased progressively with increasing genistein dose (28.9% ± 1.2%, 33.8% ± 1.6% and 37.7% ± 1.2%) . The positive rate of bax protein was increased progressively (0.9% ± 1.7%, 24.9% ± 0.8% and 29.6% ± 1.7%) by by immunohistochemical staining, with increasing dose of genistein. The density of bax mRNA increased progressively with elongation of time by RT-PCR. |
18203299 |
MMP-AC0019 |
CASP3 |
caspase 3, apoptosis-related cysteine peptidase |
836 |
Liver Cancer |
HUH7 |
- |
- |
- |
- |
Compound showed at least a 50%increase in caspase activity showing the ability of these flavonoids to induce apoptosis in HuH-7 cells |
21495101 |
MMP-AC0019 |
CASP3 |
caspase 3, apoptosis-related cysteine peptidase |
836 |
Breast Carcinoma |
Hs578T |
- |
- |
- |
- |
It induced cell apoptosis through a caspase-3-dependent pathway and mediated cell cycle arrest in the G2/M phase. The induction of apoptosis in the breast cancer cell lines by genistein isoflavone confirmed by detection of increased caspase-3 activity in the isoflavone-treated HS578T and MDA-MB-231 cells. |
19013426 |
MMP-AC0019 |
CASP3 |
caspase 3, apoptosis-related cysteine peptidase |
836 |
Breast Carcinoma |
MDA-MB-231 |
- |
- |
- |
- |
It induced cell apoptosis through a caspase-3-dependent pathway and mediated cell cycle arrest in the G2/M phase. The induction of apoptosis in the breast cancer cell lines by genistein isoflavone confirmed by detection of increased caspase-3 activity in the isoflavone-treated HS578T and MDA-MB-231 cells. |
19013426 |
MMP-AC0019 |
CDK1 |
cyclin-dependent kinase 1 |
983 |
Bladder Cancer |
J82 |
- |
- |
- |
- |
A dose-dependent inhibition of the cdc2 kinase activity, corresponding to the observed cell cycle arrest effect, was observed in most bladder cancer cell line |
10656454 |
MMP-AC0019 |
CDK1 |
cyclin-dependent kinase 1 |
983 |
Bladder Cancer |
T24 |
- |
- |
- |
- |
A dose-dependent inhibition of the cdc2 kinase activity, corresponding to the observed cell cycle arrest effect, was observed in most bladder cancer cell line |
10656454 |
MMP-AC0019 |
CDK1 |
cyclin-dependent kinase 1 |
983 |
Uroepithelials |
HT-1376 |
- |
- |
- |
- |
A dose-dependent inhibition of the cdc2 kinase activity, corresponding to the observed cell cycle arrest effect, was observed in most bladder cancer cell line |
10656454 |
MMP-AC0019 |
CDK1 |
cyclin-dependent kinase 1 |
983 |
Human Melanoma |
OCM-1 |
- |
- |
- |
- |
Genistein inhibited CDK-1 by 50-70. |
11322924 |
MMP-AC0019 |
CDKN1A |
cyclin-dependent kinase inhibitor 1A (p21, Cip1) |
1026 |
Human Melanoma |
OCM-1 |
- |
- |
- |
- |
Genistein which caused a G2/M block and did not affect CDK2 activity, led to a clear increase in the content of p21CIP. |
11322924 |
MMP-AC0019 |
EGFR |
epidermal growth factor receptor |
1956 |
Breast adenocarcinoma |
MCF-7 |
0.7 µg/mL |
- |
- |
- |
Genistein is a potent inhibitor of the PTK activity of the EGF-R in vitro. It has been reported to inhibit the growth of a variety of cell lines in vitro stimulated by serum, E2, and growth factors, but a systematic study of the effects of genistein on breast cancer cells has not been reported. |
8891338 |
MMP-AC0019 |
NFKB1 |
nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 |
4790 |
Pancreatic cancer |
COLO 357 |
- |
- |
- |
- |
In vivo results were similar to in vitro findings, suggesting that the inactivation of NF-kappaB is at least one of the molecular mechanisms by which genistein potentiates gemcitabine-induced antitumor activity in experimental animal model |
16204081 |